anti hsp90ab  (Santa Cruz Biotechnology)

Journal: International Journal of Molecular Sciences

Article Title: CT2A Cell Viability Modulated by Electromagnetic Fields at Extremely Low Frequency under No Thermal Effects

doi: 10.3390/ijms21010152

Figure Lengend Snippet: Immunofluorescence analysis of HSP90 expression in CT2A exposed to a 100 µT EMF (Microscope 20×): ( A ) CT2A control group (non-exposed), nuclei staining with DAPI; ( B ) CT2A control group (non-exposed) HSP90AB expression; ( C ) merge of A and B; ( D ) CT2A after the exposure to a 100 µT EMF at 20 Hz, nuclei staining with DAPI; ( E ) CT2A after the exposure to a 100 µT EMF at 20 Hz, HSP90AB expression; ( F ) merge of D and E; ( G ) CT2A after the exposure to a 100 µT EMF at 30 Hz, nuclei staining with DAPI; ( H ) CT2A after the exposure to a 100 µT EMF at 30 Hz, HSP90AB expression; ( I ) merge of G and H; ( J ) CT2A after the exposure to a 100 µT EMF at 50 Hz, nuclei staining with DAPI; (K) CT2A after the exposure to a 100 µT EMF at 50 Hz, HSP90AB expression; ( L ) merge of A and B.

Article Snippet: The expression of heat effects was analyzed by the primary antibody anti-HSP90AB (Sigma Aldrich Ref: SAB4300541), which results in a cytoplasmic staining of the cell.

Techniques: Immunofluorescence, Expressing, Microscopy, Staining

Journal: Cell Death & Disease

Article Title: Toll-like receptor 4 modulates the cochlear immune response to acoustic injury

doi: 10.1038/cddis.2016.156

Figure Lengend Snippet: Tlr4 expression in non-organ of Corti tissues. ( a ) Constitutive expression of Tlr4 mRNA in the organ of Corti and non-organ of Corti tissues. The non-organ of Corti tissues contain the lateral wall and the basilar membrane, where immune cells reside. The dots represent individual values, and the horizontal lines represent the group mean. The expression levels are normalized using two reference genes (Rpl13a and Hsp90ab1). The average expression level of Tlr4 in the non-organ of Corti tissues was significantly higher than in the organ of Corti (Student's t test, t (6)=4.72, P =0.003). ( b ) A typical image showing Tlr4 immunoreactivity in cells beneath the basilar membrane. Arrows indicate the positive cells with strong Tlr4 immunoreactivity. ( c ) Double-labeling of F4/80, a macrophage marker protein, in the same tissue. Bar=20 μ m. ( d ) Merged image of ( b and c ). Notice that the cells with strong Tlr4 immunoreactivity are also F4/80-positive

Article Snippet: Pre-developed Hsp90ab and Rpl13a gene expression assays (Applied Biosystems) were used as endogenous controls. qRT-PCR was performed using a MyIQ two color Real-Time PCR detection system (Bio-Rad, Hercules, CA, USA).

Techniques: Expressing, Labeling, Marker